Journal: Frontiers in Genetics

Article Title: Comprehensive Analysis of Sinonasal Inverted Papilloma Expression Profiles Identifies Long Non-Coding RNA AKTIP as a Potential Biomarker

doi: 10.3389/fgene.2022.831759

Figure Lengend Snippet: Top 20 differentially expressed lncRNAs in SNIP tissues detected by microarray assay.

Article Snippet: A human lncRNA microarray (4 × 180K; v 6.0) was manufactured at the Shanghai Biochip Co., Ltd. (Shanghai, China), which contains 95,956 capture probes for 77,103 lncRNAs and 18,853 RNAs based on the most authoritative databases, such as GENCODE v21, Ensembl, LNCipedia v3.1, Lncrnadb, Noncode v4 and UCSC.

Techniques: Microarray

Journal: Frontiers in Genetics

Article Title: Comprehensive Analysis of Sinonasal Inverted Papilloma Expression Profiles Identifies Long Non-Coding RNA AKTIP as a Potential Biomarker

doi: 10.3389/fgene.2022.831759

Figure Lengend Snippet: Top 20 differentially expressed mRNAs in SNIP tissues detected by microarray assay.

Article Snippet: A human lncRNA microarray (4 × 180K; v 6.0) was manufactured at the Shanghai Biochip Co., Ltd. (Shanghai, China), which contains 95,956 capture probes for 77,103 lncRNAs and 18,853 RNAs based on the most authoritative databases, such as GENCODE v21, Ensembl, LNCipedia v3.1, Lncrnadb, Noncode v4 and UCSC.

Techniques: Microarray

Journal: Frontiers in Genetics

Article Title: Comprehensive Analysis of Sinonasal Inverted Papilloma Expression Profiles Identifies Long Non-Coding RNA AKTIP as a Potential Biomarker

doi: 10.3389/fgene.2022.831759

Figure Lengend Snippet: qRT-PCR validation of eight differentially expressed lncRNAs and three mRNAs selected by the microarray assay. Comparison of lncRNA or mRNA expression levels obtained by microarray and qRT-PCR analysis. Upregulated and downregulated lncRNAs or mRNAs are indicated by bars above and below the horizontal axis, respectively. The data from qRT-PCR was shown as mean ± standard deviation (SD).

Article Snippet: A human lncRNA microarray (4 × 180K; v 6.0) was manufactured at the Shanghai Biochip Co., Ltd. (Shanghai, China), which contains 95,956 capture probes for 77,103 lncRNAs and 18,853 RNAs based on the most authoritative databases, such as GENCODE v21, Ensembl, LNCipedia v3.1, Lncrnadb, Noncode v4 and UCSC.

Techniques: Quantitative RT-PCR, Microarray, Expressing, Standard Deviation

Journal:

Article Title: Glomeruloid Microvascular Proliferation Follows Adenoviral Vascular Permeability Factor/Vascular Endothelial Growth Factor-164 Gene Delivery

doi:

Figure Lengend Snippet: Overview Regarding Expression of Cell-Specific Markers, Basal Lamina and Extracellular Matrix Components in Normal Vessels and in Glomeruloid Bodies (GBs) at Different Stages of Their Evolution

Article Snippet: 14-31 table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody and source Normal microvessels 0 days Primitive GB 3–4 days Mature GB 7–10 days Devolving GB 14–21 days End stage >28 days Endothelium CD31; Pharmingen, San Diego CA (mAb) 14,15 + + + + + VE cadherin; Pharmingen (mAb) 16 + − − −/+ + VEGFR-2 protein; Drs. Brekken/Thorpe (pAb) + + + + + Neuropilin; Oncogene, Cambridge, MA (pAb) − − − −/+ − Pericytes NG2; Chemicon, Temecula CA (mAb) 17,18 −/+ − + + + α-SMA; Sigma, St. Louis MO (mAb clone 1A4) 19 + − −/+ + + PDGF β-receptor; Santa Cruz (mAb clone 958) 20 −/+ −/+ + + + Smooth muscle myosin; Sigma (mAb clone hSM-V) 21 −/+ − − −/+ −/+ α and γ-actin; DAKO (mAb clone HHF) 22,23 −/+ − − −/+ −/+ Calponin; Sigma (mAb clone hCP) 24,25 −/+ − − −/+ −/+ Desmin; Chemicon (mAb) −/+ − − −/+ −/+ Macrophages F4/80; Serotec, Raleigh NC (mAb) − − + + − HLA class II; DAKO (mAb clone CR3/43) 26 − − + + − Basal Lamina Entactin; Chemicon mAb (mAb) 27 + + + + + Perlecan; mAb Chemicon (mAb) 28 + + + + + Laminin; mAb Chemicon (mAb) 29 + + + + + Collagen IV; Chemicon (mAb) 30 + + + + + Extracellular matrix Fibrillar collagen, Masson trichrome stain (Sigma) + − − −/+ + Fibrin, anti-fibrinogen; DAKO (pAb) 31 − − + + −/+ Open in a separate window +, all cells/structures positive; −/+, focal expression; −, no detectable expression. mAb, monoclonal antibody; pAb, polyclonal antibody.

Techniques: Expressing, Staining